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Immunization with biodegradable nanoparticles efficiently induces cellular immunity and protects against influenza virus infection.
Knuschke, Torben; Sokolova, Viktoriya; Rotan, Olga; Wadwa, Munisch; Tenbusch, Matthias; Hansen, Wiebke; Staeheli, Peter; Epple, Matthias; Buer, Jan; Westendorf, Astrid M.
Afiliación
  • Knuschke T; Infection Immunology, Institute of Medical Microbiology, University Hospital Essen, University of Duisburg-Essen, 45122 Essen, Germany.
J Immunol ; 190(12): 6221-9, 2013 Jun 15.
Article en En | MEDLINE | ID: mdl-23667109
The ability of vaccines to induce T cell responses is crucial for preventing diseases caused by viruses or bacteria. Nanoparticles (NPs) are considered an efficient tool for inducing potent immune responses. In this study, we describe a novel vaccination approach with biodegradable calcium phosphate (CaP) NPs that serve as carrier of immunoactive TLR9 ligand (CpG) combined with a viral Ag from the influenza A virus hemagglutinin. Functionalized CaP NPs were efficiently taken up by dendritic cells in vivo and elicited a potent T cell-mediated immune response in immunized mice with high numbers of IFN-γ-producing CD4(+) and CD8(+) effector T cells. Most importantly, both i.p. and intranasal immunization with these NPs offered protection in a mouse model of influenza virus infection. This study demonstrates the great potential of CaP NPs as a novel vaccination tool that offers substantial flexibility for several infection models.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Vacunas contra la Influenza / Vacunación / Nanopartículas / Inmunidad Celular Límite: Animals Idioma: En Revista: J Immunol Año: 2013 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Vacunas contra la Influenza / Vacunación / Nanopartículas / Inmunidad Celular Límite: Animals Idioma: En Revista: J Immunol Año: 2013 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Estados Unidos