High levels of polymorphism found through cross-amplification of microsatellite loci in a Ctenomys pearsoni (Rodentia, Ctenomyidae) population.
Genet Mol Res
; 12(2): 887-91, 2013 Apr 02.
Article
en En
| MEDLINE
| ID: mdl-23613235
Ctenomys pearsoni (Pearson's tuco-tuco) is a subterranean rodent native to Uruguay. We tested the amplification pattern of 12 microsatellite loci, designed for C. sociabilis and C. haigi in a C. pearsoni population. DNA extractions were made from hair samples, and PCR amplification products were run on an ABI 3100 microcapillary gel. Eight loci were selected to form a highly polymorphic panel that could be used to efficiently screen populations of this species. In DNA from 35 tuco-tucos, the mean polymorphic information content value was 0.6536 and the mean expected heterozygosity was 0.7166. Paternity non-exclusion probabilities for seven independent loci were NE-1P = 0.0766 and NE-2P = 0.0108, and combined non-exclusion P(ID) was 6.2 x 10(-7). This panel of microsatellite loci has sufficient power to make inferences regarding group structure, mating strategies and evolutionary relationships among populations.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Polimorfismo Genético
/
Roedores
/
Repeticiones de Microsatélite
Límite:
Animals
Idioma:
En
Revista:
Genet Mol Res
Asunto de la revista:
BIOLOGIA MOLECULAR
/
GENETICA
Año:
2013
Tipo del documento:
Article
País de afiliación:
Uruguay
Pais de publicación:
Brasil