Optimization of hydrogen peroxide detection for a methyl mercaptan biosensor.
Sensors (Basel)
; 13(4): 5028-39, 2013 Apr 15.
Article
en En
| MEDLINE
| ID: mdl-23591963
Several kinds of modified carbon screen printed electrodes (CSPEs) for amperometric detection of hydrogen peroxide (H2O2) are presented in order to propose a methyl mercaptan (MM) biosensor. Unmodified, carbon nanotubes (CNTs), cobalt phthalocyanine (CoPC), Prussian blue (PB), and Os-wired HRP modified CSPE sensors were fabricated and tested to detect H2O2, applying a potential of +0.6 V, +0.6 V, +0.4 V, -0.2 V and -0.1 V (versus Ag/AgCl), respectively. The limits of detection of these electrodes for H2O2 were 3.1 µM, 1.3 µM, 71 nM, 1.3 µM, 13.7 nM, respectively. The results demonstrated that the Os-wired HRP modified CSPEs gives the lowest limit of detection (LOD) for H2O2 at a working potential as low as -0.1 V. Os-wired HRP is the optimum choice for establishment of a MM biosensor and gives a detection limit of 0.5 µM.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Compuestos de Sulfhidrilo
/
Técnicas Biosensibles
/
Peróxido de Hidrógeno
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Sensors (Basel)
Año:
2013
Tipo del documento:
Article
País de afiliación:
China
Pais de publicación:
Suiza