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A predictive microarray-based biomarker for early detection of Alzheimer's disease intended for clinical diagnostic application.
Calciano, Margaret; Lemarié, Jean Christophe; Blondiaux, Elodie; Einstein, Richard; Fehlbaum-Beurdeley, Pascale.
Afiliación
  • Calciano M; Exonhit, Inc., Gaithersburg, MD, USA.
Biomarkers ; 18(3): 264-72, 2013 May.
Article en En | MEDLINE | ID: mdl-23557129
OBJECTIVE: Microarray-based signatures for clinical application are often plagued by processing variability or batch effects that compromise the robustness of the test performance. METHODS: A splice variant array-based signature for early detection of Alzheimer's disease (AD) was developed using 315 AD or normal subjects processed in three disparate microarray batches. RESULTS: A modified top scoring pair classifier using the signature, is robust to batch effects and outperforms other common classifiers, with sensitivity and specificity of 88.3% (95% CI:81.2%, 93.4%) and 88.9% (95% CI:65.3%, 98.6%), respectively, on an independent cohort. CONCLUSIONS: This splice-variant array-based signature shows promise for clinical diagnostic use in AD.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Algoritmos / ARN / Análisis de Secuencia por Matrices de Oligonucleótidos / Enfermedad de Alzheimer / Transcriptoma Tipo de estudio: Diagnostic_studies / Prognostic_studies / Risk_factors_studies / Screening_studies Límite: Aged / Aged80 / Female / Humans / Male Idioma: En Revista: Biomarkers Asunto de la revista: BIOQUIMICA Año: 2013 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Algoritmos / ARN / Análisis de Secuencia por Matrices de Oligonucleótidos / Enfermedad de Alzheimer / Transcriptoma Tipo de estudio: Diagnostic_studies / Prognostic_studies / Risk_factors_studies / Screening_studies Límite: Aged / Aged80 / Female / Humans / Male Idioma: En Revista: Biomarkers Asunto de la revista: BIOQUIMICA Año: 2013 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido