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DAXX silencing suppresses mouse ovarian surface epithelial cell growth by inducing senescence and DNA damage.
Pan, Wei-Wei; Yi, Fa-Ping; Cao, Li-Xian; Liu, Xiao-Man; Shen, Zhong-Fei; Bu, You-Quan; Xu, Ying; Fan, Heng-Yu; Song, Fang-Zhou.
Afiliación
  • Pan WW; Department of Biochemistry and Molecular Biology, Chongqing Medical University, Chongqing, China.
Gene ; 526(2): 287-94, 2013 Sep 10.
Article en En | MEDLINE | ID: mdl-23542781
Mouse ovarian surface epithelium (OSE) is a single layer of cubodial epithelial cells that covers the ovary surface and is involved in regulating the secretion and transport of 17ß-hydroxysteroid dehydrogenase. Recently, OSE cells have attracted particular interest as a major source of ovarian cancer. Death-associated protein DAXX along with PML (promyelocytic leukemia protein) nuclear bodies (PML-NBs) reportedly play roles in transcriptional regulation and apoptosis. However, little is known regarding a role for DAXX in mOSE cells. In this study, we both over-expressed DAXX and depleted DAXX in primary mOSE cells. We found that Daxx deletion accelerated senescence in a p53/p21-dependent manner and promoted DNA damage by interacting with PML bodies without affecting cell cycle progression. These results suggest that DAXX may transform mOSE cells to an ovarian oncogenic phenotype and may be an anti-cancer target.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ovario / Daño del ADN / Proteínas Nucleares / Proteínas Portadoras / Senescencia Celular / Silenciador del Gen / Péptidos y Proteínas de Señalización Intracelular / Células Epiteliales Límite: Animals / Female / Humans Idioma: En Revista: Gene Año: 2013 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ovario / Daño del ADN / Proteínas Nucleares / Proteínas Portadoras / Senescencia Celular / Silenciador del Gen / Péptidos y Proteínas de Señalización Intracelular / Células Epiteliales Límite: Animals / Female / Humans Idioma: En Revista: Gene Año: 2013 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos