Isolation and identification of environmental mycobacteria in the waters of a hemodialysis center.
Curr Microbiol
; 67(1): 107-11, 2013 Jul.
Article
en En
| MEDLINE
| ID: mdl-23463515
The use of poorly treated water during hemodialysis may lead to contamination with nontuberculous mycobacteria (NTM). This study aimed to isolate and identify NTM species in the water of a Brazilian hemodialysis center. We collected 210 samples of water from the hydric system of the unit (post-osmosis system, hemodialysis rooms, reuse system, and hemodialysis equipment) and from the municipal supply network; we isolated the NTM by a classic microbiological technique and identified them by the PCR restriction enzyme pattern of the hsp65 gene (PRA). Fifty-one (24.3 %) of the collected samples tested positive for NTM; both the municipal supply network (2 samples, 3.2 %) and the hydric system of the hemodialysis center (49 samples, 96.1 %) contained NTM. We isolated and identified potentially pathogenic bacteria such as Mycobacterium lentiflavum (59.0 %) and M. kansasii (5.0 %), as well as rarely pathogenic bacteria like M. gordonae (24.0 %), M. gastri (8.0 %), and M. szulgai (4.0 %). The ability of NTM to cause diseases is well documented in the literature. Therefore, the identification of NTM in the water of a Brazilian hemodialysis center calls for more effective water disinfection procedures in this unit.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Microbiología del Agua
/
Micobacterias no Tuberculosas
Tipo de estudio:
Diagnostic_studies
País/Región como asunto:
America do sul
/
Brasil
Idioma:
En
Revista:
Curr Microbiol
Año:
2013
Tipo del documento:
Article
País de afiliación:
Brasil
Pais de publicación:
Estados Unidos