Prediction and characterization of the linear IgE epitopes for the major soybean allergen ß-conglycinin using immunoinformatics tools.
Food Chem Toxicol
; 56: 254-60, 2013 Jun.
Article
en En
| MEDLINE
| ID: mdl-23454299
The α subunit of ß-conglycinin is a major allergen in soybean. The objective of this study was to predict and identify the linear immunoglobulin (Ig)E epitopes of the soybean α subunit of ß-conglycinin. Three immunoinformatics tools were used to predict the potential epitopes and were confirmed by dot-blot inhibition using sera from soybean allergic subjects. As a result, 15 peptides were predicted and assembled by solid-phase synthesis. Eleven epitopes were identified by the dot-blot inhibition test. Moreover, peptide 3 had IgE binding capability with all sera(5/5) tested, while peptide 1, 4, 6, 8 and12 could bind to 4/5 of the sera samples. Secondary structure prediction of peptide 3 and circular dichroism test validated that the structure of peptide 3 was a random coil.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Glycine max
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Inmunoglobulina E
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Alérgenos
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Proteínas de Soja
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Antígenos de Plantas
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Proteínas de Almacenamiento de Semillas
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Globulinas
Tipo de estudio:
Prognostic_studies
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Risk_factors_studies
Límite:
Adult
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Female
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Humans
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Male
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Middle aged
Idioma:
En
Revista:
Food Chem Toxicol
Año:
2013
Tipo del documento:
Article
País de afiliación:
China
Pais de publicación:
Reino Unido