SCE induction and cell-cycle delay by toxaphene.
Mutat Res
; 230(1): 29-33, 1990 May.
Article
en En
| MEDLINE
| ID: mdl-2342495
Toxaphene is genotoxic in mammalian cell systems and also inhibits cell replication. It was therefore used to investigate possible masking of SCE induction due to cell-cycle delay. In this study, toxaphene-treated Chinese hamster lung (Don) cells exhibited a dose-dependent decrease in cell-cycle progression compared with untreated cells. At high, nontoxic toxaphene levels (15 micrograms/ml), cell cycling also slowed as the toxaphene treatment time was increased. Toxaphene induced significantly higher numbers of SCEs in treated cells, demonstrating a dose- and treatment time-relationship. Slopes of dose-response curves were 0.29, 0.43 and 0.77 SCE/micrograms toxaphene for 20.5 h, 24.5 h and 28.5 h incubation, respectively. There were no changes in SCE values in control cultures even when slower dividing cells were sampled e.g. at longer incubation times. Thus, higher SCE values in Chinese hamster cells were not associated per se with slower or more delayed cells. The results demonstrate that longer toxaphene treatment times were not necessary for obtaining sufficient harlequin-stained cells for SCE analysis, but that higher numbers of SCEs occurred in slower dividing cells, following prolonged incubation of cultures treated with toxaphene.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Intercambio de Cromátides Hermanas
/
Toxafeno
/
Ciclo Celular
/
Insecticidas
Límite:
Animals
Idioma:
En
Revista:
Mutat Res
Año:
1990
Tipo del documento:
Article
País de afiliación:
Australia
Pais de publicación:
Países Bajos