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HLA class II-restricted binding of muramyl peptides to B lymphocytes of normal and narcoleptic subjects.
Silverman, D H; Sayegh, M H; Alvarez, C E; Johnson, T S; Milford, E L; Karnovsky, M L.
Afiliación
  • Silverman DH; Harvard Medical School, Boston, Massachusetts.
Hum Immunol ; 27(3): 145-54, 1990 Mar.
Article en En | MEDLINE | ID: mdl-2341300
The propensity for narcolepsy, a clinical sleep disorder of unknown etiology, is virtually totally included within the HLA-DR2,DQw1 (DRw15,DQw6) phenotype. The disorder is characterized by decreased sleep latency, early onset of rapid eye movement sleep, and a paucity of nocturnal slow-wave sleep. Muramyl peptides, naturally occurring bacterial cell wall peptidoglycans, potently enhance the duration and amplitude of slow-wave sleep in animals, bind to murine mononuclear cells, and exhibit a major histocompatibility complex-restricted immunoadjuvant effect in mice. We examined the binding of muramyl peptides to peripheral blood mononuclear leukocytes of HLA-typed normal (n = 13) and narcoleptic (n = 10) subjects. Muramyl peptides bound specifically and with high affinity to normal B- but not T-lymphocyte-enriched preparations. There was no significant specific binding to B-cell-enriched preparations from narcoleptic patients. Furthermore, B-lymphocyte-enriched preparations of normal individuals who had the HLA-DR2,DQw1 phenotype (n = 8) exhibited a lower level of specific binding than those of normals who did not have this phenotype (n = 5, p less than 0.001). These observations are an additional indication of the relevance of muramyl peptides to slow-wave sleep and provide a basis for a better understanding of the relation between narcolepsy and the MHC at the biochemical level.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Linfocitos B / Antígenos de Histocompatibilidad Clase II / Acetilmuramil-Alanil-Isoglutamina / Narcolepsia Límite: Adult / Female / Humans / Male / Middle aged Idioma: En Revista: Hum Immunol Año: 1990 Tipo del documento: Article Pais de publicación: Estados Unidos
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Linfocitos B / Antígenos de Histocompatibilidad Clase II / Acetilmuramil-Alanil-Isoglutamina / Narcolepsia Límite: Adult / Female / Humans / Male / Middle aged Idioma: En Revista: Hum Immunol Año: 1990 Tipo del documento: Article Pais de publicación: Estados Unidos