Abnormal platelet amyloid-ß protein precursor (AßPP) metabolism in Alzheimer's disease: identification and characterization of a new AßPP isoform as potential biomarker.
J Alzheimers Dis
; 35(2): 285-95, 2013.
Article
en En
| MEDLINE
| ID: mdl-23388171
Previous findings demonstrated an altered pattern of amyloid-ß protein precursor (AßPP) expression in platelets of Alzheimer's disease (AD) patients compared with either healthy control subjects or patients with non-Alzheimer-type dementia. In an attempt to explore the diagnostic potential of platelet AßPP metabolism, we have generated monoclonal antibodies directed to the N-terminal part of AßPP. We have observed two different antibody recognition patterns of AßPP: one resembling previously described 130 kDa and 105 kDa species and a novel AßPP 115 kDa form. This form was significantly increased in platelets of the mild cognitive impairment and AD group as compared to control subjects. The abundance of AßPP 115 kDa species correlated with the previously described AßPP 130/105 kDa ratio as well as with Mini-Mental State Examination score. Despite the inability of these particular monoclonal antibodies to recognize native forms of AßPP, identification of a new AßPP isoform in platelets as a potential AD biomarker can provide an additional tool for the development of a reliable diagnostic test to detect preclinical stages of AD.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Plaquetas
/
Biomarcadores
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Precursor de Proteína beta-Amiloide
/
Enfermedad de Alzheimer
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Límite:
Aged
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Animals
/
Female
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Humans
/
Male
Idioma:
En
Revista:
J Alzheimers Dis
Asunto de la revista:
GERIATRIA
/
NEUROLOGIA
Año:
2013
Tipo del documento:
Article
País de afiliación:
Suecia
Pais de publicación:
Países Bajos