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The Effect of Elephantopus scaber L. on Liver Regeneration after Partial Hepatectomy.
Tsai, Chin-Chuan; Wu, Jia-Ping; Lin, Yueh-Min; Yeh, Yu-Lan; Ho, Tsung-Jung; Kuo, Chia-Hua; Tzang, Bor-Show; Tsai, Fuu-Jen; Tsai, Chang-Hai; Huang, Chih-Yang.
Afiliación
  • Tsai CC; School of Chinese Medicine for Post-Baccalaureate, I-Shou University, No 91. Hsueh-Shih Road, Taichung, R.O.C., Kaohsiung 40402, Taiwan ; Chinese Medicine Department, E-Da Hospital, Kaohsiung, Taiwan.
Article en En | MEDLINE | ID: mdl-23365606
Liver regeneration after partial hepatectomy (PHx) is a physiological response for maintaining homeostasis. The aim of this study is to investigate effects of Elephantopus scaber L.- (ESL-) induced liver regeneration on growth factors (HGF and IGF-1), cell cycle regulation, and apoptosis suppressed. In this study, we fed five Chinese medicinal herbs (1 g/kg/day), Codonopsis pilosula (CP, Dangshen), Salvia miltiorrhiza Bunge (SMB, Danshen,), Bupleurum kasi (BK, Chaihu), Elephantopus scaber L. (ESL, Teng-Khia-U), and Silymarin (Sm, 25 mg/kg) for 7 days to male Spraue-Dawley rats. Then surgical 2/3 PHx was conducted and liver regeneration mechanisms were estimated on the following 24 hrs and 72 hrs. The activities of growth factors (HGF and IGF-I) and cell cycle proteins were measured by Western blot and RT-PCR. Histological analysis and apoptosis were detected by H&E stain and TUNEL. The results showed that extraction of Elephantopus scaber L. (ESL) and Silymarin (Sm, positive control) were increased protein expression levels of HGF and IGF-1 which leads into cell cycle. These results suggest that the ESL plays a crucial role in cell cycle-induced liver regeneration and apoptosis. These results suggested that the ESL plays a crucial role in cell cycle-induced liver regeneration and suppressed hepatocytes apoptosis.

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Evid Based Complement Alternat Med Año: 2013 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Evid Based Complement Alternat Med Año: 2013 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Estados Unidos