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Semen quality and sperm function loss by hypercholesterolemic diet was recovered by addition of olive oil to diet in rabbit.
Saez Lancellotti, Tania E; Boarelli, Paola V; Romero, Aida A; Funes, Abi K; Cid-Barria, Macarena; Cabrillana, María E; Monclus, María A; Simón, Layla; Vicenti, Amanda E; Fornés, Miguel W.
Afiliación
  • Saez Lancellotti TE; Laboratorio de Investigaciones Andrológicas de Mendoza (LIAM), Instituto de Histología y Embriología (IHEM), Facultad de Ciencias Médicas, Universidad Nacional de Cuyo, Centro Científico Tecnológico (CCT) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Mendoza, Argentina.
PLoS One ; 8(1): e52386, 2013.
Article en En | MEDLINE | ID: mdl-23326331
Fat increment (0.05% cholesterol, chol) in standard diet promoted a significant increase in serum and sperm membrane chol, which ultimately altered membrane-coupled sperm specific functions: osmotic resistance, acrosomal reaction, and sperm capacitation in White New Zealand rabbits. These changes were also associated with a reduction in motility percentage and appearance of abnormal sperm morphology. The present study was carried out to evaluate the effect of dietary olive oil (OO, 7% v/w) administration to several male hypercholesterolemic rabbits (hypercholesterolemic rabbits, HCR) with altered fertility parameters. These HCR males were achieved by feeding normal rabbits with a high-fat diet (0.05% chol). HCR were associated with a modest non-significant increase in body weight (standard diet, 4.08±0.17 Kg, versus high-fat diet, 4.37±0.24 Kg). Hypercholesterolemic rabbits presented a marked decrease in semen volume, sperm cell count, and percentage of sperm motility, associated with a significant increase in sperm cell abnormalities. Moreover, sperm capacitation measured by the characteristic phosphorylated protein pattern in and induced acrosomal reaction were also altered suggesting sperm dysfunction. However, the administration of OO (for 16 weeks) to rabbits that were fed with 50% of the high-fat diet normalized serum chol. Curiously, OO supply succeeded to attenuate the seminal and sperm alterations observed in HCR group. Administration of OO alone did not cause any significant changes in above mentioned parameters. These data suggest that OO administration to HCR male rabbits recovers the loss of semen quality and sperm functionality.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Espermatozoides / Aceites de Plantas / Análisis de Semen / Hipercolesterolemia Tipo de estudio: Etiology_studies Límite: Animals Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2013 Tipo del documento: Article País de afiliación: Argentina Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Espermatozoides / Aceites de Plantas / Análisis de Semen / Hipercolesterolemia Tipo de estudio: Etiology_studies Límite: Animals Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2013 Tipo del documento: Article País de afiliación: Argentina Pais de publicación: Estados Unidos