Effect of tubulin binding and self-association on the near-ultraviolet circular dichroic spectra of colchicine and analogues.
Biochemistry
; 29(7): 1869-75, 1990 Feb 20.
Article
en En
| MEDLINE
| ID: mdl-2331468
Near-UV circular dichroic (CD) spectra of three colchicine analogues that differ at the C-10 position have been obtained in the presence and absence of tubulin. All three colchicine analogues show dramatic alterations in the low-energy near-UV CD band upon tubulin binding that cannot be mimicked by solvent, but in no event does the rotational strength of the CD band decrease to nearly zero as in the case of colchicine [Detrich, H. W., III, Williams, R. C., Jr., Macdonald, T. L., & Puett, D. (1981) Biochemistry 20, 5999-6005]. The effect of self-association of colchicine and one of the C-10 analogues, thiocolchicine, on the near-UV CD band was also investigated. A qualitative similarity was seen between the near-UV CD spectra of colchicine and thiocolchicine dimers and the spectra of these molecules bound to tubulin. These observations support the previous suggestion that ligands bound to the colchicine site on tubulin may be interacting with an aromatic amino acid in the colchicine binding site [Hastie, S. B., & Rava, R. P. (1989) J. Am. Chem. Soc. 110, 6993-7001].
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Tubulina (Proteína)
/
Colchicina
Tipo de estudio:
Qualitative_research
/
Risk_factors_studies
Límite:
Animals
Idioma:
En
Revista:
Biochemistry
Año:
1990
Tipo del documento:
Article
Pais de publicación:
Estados Unidos