Chiral capillary electrophoresis-mass spectrometry.
Methods Mol Biol
; 970: 429-41, 2013.
Article
en En
| MEDLINE
| ID: mdl-23283794
Capillary electrophoresis-mass spectrometry (CE-MS) is a powerful analytical tool, especially in the case of chiral separations, due to the fact that it combines the high efficiency, short analysis time, and versatility of the CE with the sensitivity, selectivity, and the capacity for the identification of unknown chiral compounds offered by MS detection. This chapter describes three methodologies enabling the chiral separation of cationic and anionic compounds using different strategies, illustrating the most employed approaches used in chiral CE-MS. The first methodology uses the partial filling technique for the enantioseparation of a cationic compound using a neutral cyclodextrin. Secondly, the enantioseparation of a cationic compound using low concentrations of a neutral cyclodextrin under acidic conditions is described. Finally, a methodology for the chiral separation of an anionic compound employing low concentrations of a native cyclodextrin under basic conditions is illustrated.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Espectrometría de Masas
/
Electroforesis Capilar
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2013
Tipo del documento:
Article
País de afiliación:
España
Pais de publicación:
Estados Unidos