[Oligomerization of site-specific nicking endonuclease BspD6I at high protein concentrations].

Sekerina, S A; Grishin, A V; Riazanova, A Iu; Artiukh, R I; Rogulin, E A; Iunusova, A K; Oretskaia, T S; Zheleznaia, L A; Kubareva, E A

Sekerina, S A; Grishin, A V; Riazanova, A Iu; Artiukh, R I; Rogulin, E A; Iunusova, A K; Oretskaia, T S; Zheleznaia, L A; Kubareva, E A.

Bioorg Khim ; 38(4): 431-8, 2012.

Article en Ru | MEDLINE | ID: mdl-23189557

RESUMEN

Ability of site-specific nickase BspD6I (Nt.BspD6I) to oligomerize at concentrations > or = 0.5 microM (> or = 0.035 mg/mL) is studied. Three states of Nt.BspD6I are registered via electrophoretic studies both in the presence and in the absence of DNA. Estimation of their molecular mass allows assigning them as a monomer, a dimer and a trimer. Both dimeric and monomeric Nt.BspD6I are shown to hydrolyze its DNA substrate with the identical specificity. Calculation of the electrostatic potential distribution on the Nt.BspD6I globule surface shows that the protein molecule is a dipole. The Nt. BspD6I oligomeric forms are likely to be the result of ionic protein interactions.

Asunto(s)

Proteínas de Unión al ADN/química; Desoxirribonucleasa I/química; Estructura Terciaria de Proteína; Bacillus/enzimología; ADN/química; Multimerización de Proteína

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Estructura Terciaria de Proteína / Desoxirribonucleasa I / Proteínas de Unión al ADN Idioma: Ru Revista: Bioorg Khim Año: 2012 Tipo del documento: Article Pais de publicación: Rusia

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