Suppression of non-small cell lung cancer proliferation and tumorigenicity by DENND2D.
Lung Cancer
; 79(2): 104-10, 2013 Feb.
Article
en En
| MEDLINE
| ID: mdl-23182661
DENND2D was identified as being down-regulated in lung cancer using a lung cancer low-expression suppression subtractive hybridization (SSH) library. In this study, DENND2D down-regulation has been observed not only in non-small cell lung cancer (NSCLC) cell lines and lung squamous cell carcinoma (SCC) tissues, but also in immortalized human bronchial epithelial (IHBE) cell lines and precancerous lesions, indicating that the down-regulation of DENND2D may be an early event in lung cancer. The relative DNA copy number and mRNA and protein expression levels of DENND2D were determined in vitro, and they revealed a complicated regulatory network at the genomic, transcriptional and translational levels. Over-expression of DENND2D significantly suppressed the proliferation of NSCLC cells in vitro and in vivo by inducing apoptosis. These results indicate that DENND2D might function as a tumor suppressor-like gene to prevent the survival and expansion of cells with genetic damage through apoptosis mechanism, and absence of DENND2D might play a permissive role, as an early event, in tumorigenesis.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Carcinoma de Células Escamosas
/
Adenocarcinoma
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Regulación Neoplásica de la Expresión Génica
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Carcinoma de Pulmón de Células no Pequeñas
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Proteínas Supresoras de Tumor
/
Neoplasias Pulmonares
Tipo de estudio:
Prognostic_studies
Límite:
Adult
/
Aged
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Animals
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Female
/
Humans
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Male
/
Middle aged
Idioma:
En
Revista:
Lung Cancer
Asunto de la revista:
NEOPLASIAS
Año:
2013
Tipo del documento:
Article
Pais de publicación:
Irlanda