[Sequence-specificity of protein-oligonucleotide interactions and development for protein isolation using sorptive medium with immobilized protein-specific oligonucleotides].
Mol Biol (Mosk)
; 46(4): 663-71, 2012.
Article
en Ru
| MEDLINE
| ID: mdl-23113356
Sequence-specificity of binding of ribonuclease binase to oligodeoxyribonucleotides immobilized in biochip gel pads was studied. Binding constants for the complexes between binase and selected oligonucleotides were measured. Oligodeoxyribonucleotides GAGAGAG and GAGAGAGAG were found to be high-specific in interaction with binase. These oligonucleotides were used as molecular probes for immobilization in a sorptive medium for subsequent isolation and concentrating of binase from diluted water solutions. Volume capacity of the developed sorptive mediums containing immobilized oligodeoxyribonucleotides GAGAGAG and GAGAGAGAG was found to be 2.6 and 2.3 mg of binase per 1 mL of sorbate correspondingly. After the procedure of affinity chromatography and elution ofbinase from sorptive medium the protein showed the same affinity of binding to oligonucleotides as the initial sample.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Análisis por Matrices de Proteínas
/
Proteínas de Unión al ADN
/
Endorribonucleasas
Límite:
Animals
/
Humans
Idioma:
Ru
Revista:
Mol Biol (Mosk)
Año:
2012
Tipo del documento:
Article
Pais de publicación:
Rusia