[Relationship between the characteristics of the human sperm movement and their attachment to the zona pellucida]. / Relation entre les caractéristiques du mouvement des spermatozoïdes humains et leur fixation à la zone pellucide.
J Gynecol Obstet Biol Reprod (Paris)
; 42(1): 49-55, 2013 Feb.
Article
en Fr
| MEDLINE
| ID: mdl-23107494
OBJECTIVE: Our objective was to study the relationships between the characteristics of the movement of sperm and their attachment to the zona pellucida. MATERIALS AND METHODS: Study related to 47 patients candidates with an IVF protocol. The puncture of the ovocytes was carried out under general anesthesia under echography control after stimulated cycles using association is the citrate of clomifene and the HMG or the busereline and the HMG. Study of the mobility of the spermatozoa was carried out thanks to a videomicrography computer-assisted before and after treatment of sperm in Percoll. The setting in contact of the spermatozoa with the ovocytes having given failures of in vitro fertilization and beforehand preserved in a saline solution was carried out during 90 minutes with 37 °C, air-CO2 with 5%. After incubation, the spermatozoa fixed at the zona pellucida, are counted thanks to a meter with hand. RESULTS: The study of the test of fixing of the spermatozoa at the zona pellucida showed that the interaction spermatozoa/ovocytes is correlated positively with the parameters of the mobility analyzed by videomicrography computer-assisted: Vcl, Vsl and the ALH. CONCLUSION: The performance of the test requires a preliminary epididymal maturation of the spermatozoa in particular the ontogenesis of the spermatic sites of recognition for the zona pellucida. A deterioration of this stage of maturation led to failures of recognition and fixing at the zona pellucida. The test can predict the outcome of IVF and towards the good technique of medical assistance to procreation (AMP).
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Motilidad Espermática
/
Interacciones Espermatozoide-Óvulo
/
Espermatozoides
/
Zona Pelúcida
/
Infertilidad Masculina
Tipo de estudio:
Etiology_studies
/
Guideline
/
Prognostic_studies
/
Risk_factors_studies
Límite:
Female
/
Humans
/
Male
Idioma:
Fr
Revista:
J Gynecol Obstet Biol Reprod (Paris)
Año:
2013
Tipo del documento:
Article
País de afiliación:
Túnez
Pais de publicación:
Francia