Intracellular labeling and quantification process by magnetic resonance imaging using iron oxide magnetic nanoparticles in rat C6 glioma cell line.
Einstein (Sao Paulo)
; 10(2): 216-21, 2012.
Article
en En, Pt
| MEDLINE
| ID: mdl-23052458
OBJECTIVE: To assess intracellular labeling and quantification by magnetic resonance imaging using iron oxide magnetic nanoparticles coated with biocompatible materials in rat C6 glioma cells in vitro. These methods will provide direction for future trials of tumor induction in vivo as well as possible magnetic hyperthermia applications. METHODS: Aminosilane, dextran, polyvinyl alcohol, and starch-coated magnetic nanoparticles were used in the qualitative assessment of C6 cell labeling via light microscopy. The influence of the transfection agent poly-L-lysine on cellular uptake was examined. The quantification process was performed by relaxometry analysis in T1 and T2weighted phantom images. RESULTS: Light microscopy revealed that the aminosilane-coated magnetic nanoparticles alone or complexed with poly-L-lysine showed higher cellular uptake than did the uncoated magnetic particles. The relaxivities of the aminosilane-coated magnetic nanoparticles with a hydrodynamic diameter of 50nm to a 3-T field were r1=(6.1±0.3)×10(-5) ms-1mL/µg, r2=(5.3±0.1)× 10(-4) ms(-1)mL/µg, with a ratio of r2 / r1 â
9. The iron uptake in the cells was calculated by analyzing the relaxation rates (R1 and R2) using a mathematical relationship. CONCLUSIONS: C6 glioma cells have a high uptake efficiency for aminosilane-coated magnetic nanoparticles complexed with the transfection agent poly-L-lysine. The large ratio r2 / r1 â
9 indicates that these magnetic nanoparticles are ideal for quantification by magnetic resonance imaging with T2-weighted imaging techniques.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Imagen por Resonancia Magnética
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Compuestos Férricos
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Nanopartículas de Magnetita
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Rastreo Celular
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Glioma
Tipo de estudio:
Qualitative_research
Límite:
Animals
Idioma:
En
/
Pt
Revista:
Einstein (Sao Paulo)
Año:
2012
Tipo del documento:
Article
País de afiliación:
Brasil
Pais de publicación:
Brasil