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Relevance of O-acetyl and phosphoglycerol groups for the antigenicity of Streptococcus pneumoniae serotype 18C capsular polysaccharide.
Chang, Janoi; Serrano, Yohanna; Garrido, Raine; Rodríguez, Laura M; Pedroso, Jessy; Cardoso, Felix; Valdés, Yury; García, Dagmar; Fernández-Santana, Violeta; Verez-Bencomo, Vicente.
Afiliación
  • Chang J; Center for Biomolecular Chemistry, Playa, Havana, Cuba. janoi.chang@cqb.cu
Vaccine ; 30(49): 7090-6, 2012 Nov 19.
Article en En | MEDLINE | ID: mdl-23036500
Capsular polysaccharides are important virulence factors of Streptococcus pneumoniae. The polysaccharide has been used as a component of vaccines against pneumococcal diseases either as plain polysaccharide or better conjugated to a protein. The last one is the vaccine of choice to target child protection. The immune responses depend on several polysaccharide physicochemical properties that can be affected during either purification or modification in the case of conjugate vaccines. In serotype 18C, the repeating unit has a complex structure having a branched pentasaccharide with two apparently labile subtituents: glycerol-phosphate and O-acetyl group. The loss of these groups may potentially reduce the ability of the 18C polysaccharide to induce the desired immune response. Therefore, the relationship of both groups with the antigenicity and immunogenicity of 18C capsular polysaccharide is explored. It is shown that glycerol-phosphate must be preserved for conserving adequate antigenicity of the 18C capsular polysaccharide. At the same time, it was proved that O-acetyl groups do not play any role for the antigenicity and immunogenicity.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polisacáridos Bacterianos / Streptococcus pneumoniae / Antígenos Bacterianos Límite: Animals / Humans Idioma: En Revista: Vaccine Año: 2012 Tipo del documento: Article País de afiliación: Cuba Pais de publicación: Países Bajos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polisacáridos Bacterianos / Streptococcus pneumoniae / Antígenos Bacterianos Límite: Animals / Humans Idioma: En Revista: Vaccine Año: 2012 Tipo del documento: Article País de afiliación: Cuba Pais de publicación: Países Bajos