[Interaction of bromophenol blue with serum albumin: criteria for using dyes for assessing the state and quantity of protein]. / Vzaimodeistvie bromfenolovogo sinego s syvorotochnym al'buminom: kriterii ispol'zovaniia krasiteliia dliia otsenki sostoianiia i kolichestva belka.
Mol Biol (Mosk)
; 24(5): 1211-8, 1990.
Article
en Ru
| MEDLINE
| ID: mdl-2290419
The optical properties of the complexes of the pH-dependent dye bromophenol blue (BPB) with human serum albumin were investigated by the spectrophotometric method. The solvatochromic longwave displacement of bound BPB-2 absorption and BPB-1/BPB-2 redistribution were shown to form the optical signal of complexes. Because of the distortion of the bound BPB-2 signal its quantity was determined as delta A630 = A630 - A660 and the use of lambda max as structural parameter was limited to low pH less than or equal to 3. The conclusion was made that BPB is inapplicable as a structural probe on account of low structural dependence of delta A630 and pH-limitation of lambda max used. The maximal absorption delta Amax = Amax - A660 and its structural independence were obtained in the region of 70-100% occupation of the dye-binding centers of the protein. It is the optimal conditions for the quantitative determination of protein. After maximal dye binding (15-16 molecules of BPB per 1 molecule of albumin) the aggregation and precipitation of the complexes occurred.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Azul de Bromofenol
/
Albúmina Sérica
Límite:
Humans
Idioma:
Ru
Revista:
Mol Biol (Mosk)
Año:
1990
Tipo del documento:
Article
Pais de publicación:
Rusia