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Design of a novel gut bacterial adhesion model for probiotic applications.
Rodes, Laetitia; Coussa-Charley, Michael; Marinescu, Daniel; Paul, Arghya; Fakhoury, Marc; Abbasi, Sana; Khan, Afshan; Tomaro-Duchesneau, Catherine; Prakash, Satya.
Afiliación
  • Rodes L; Biomedical Technology and Cell Therapy Research Laboratory, Faculty of Medicine, Department of Biomedical Engineering and Artificial Cells and Organs Research Centre, McGill University, Duff Medical Building, Montreal, Quebec, Canada.
Artif Cells Nanomed Biotechnol ; 41(2): 116-24, 2013 Apr.
Article en En | MEDLINE | ID: mdl-22889228
A new gut bacterial adhesion model has been developed. For this, a continuous-flow bioreactor packed with bacteria-coated beads was designed to simulate the gut lining and other features. In vitro model efficacy shows successful bacterial cell gut adhesions: bacterial adhesion was higher with mucin-alginate compared to controls. In feasibility study, adhesion of Lactobacillus fermentum NCIMB 5221 and Lactobacillus reuteri NCIMB 701359 was investigated for their metabolic activities for bile salt. Bile salt hydrolase (BSH)-active Lactobacillus reuteri exerted higher activity than non-BSH-active L. fermentum. This model has potential use in gut health, probiotic, bacterial cell gut adhesion and other delivery applications.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Bacterianas / Ácidos y Sales Biliares / Probióticos / Limosilactobacillus fermentum / Limosilactobacillus reuteri / Amidohidrolasas / Modelos Anatómicos Límite: Humans Idioma: En Revista: Artif Cells Nanomed Biotechnol Año: 2013 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Bacterianas / Ácidos y Sales Biliares / Probióticos / Limosilactobacillus fermentum / Limosilactobacillus reuteri / Amidohidrolasas / Modelos Anatómicos Límite: Humans Idioma: En Revista: Artif Cells Nanomed Biotechnol Año: 2013 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Reino Unido