Cationic polyelectrolyte copolymer modified polyurethane foam for flow injection preconcentration and separation of trace amounts of ß-lactam antibiotics.
Talanta
; 97: 513-20, 2012 Aug 15.
Article
en En
| MEDLINE
| ID: mdl-22841116
A more sensitive flow injection preconcentration method has been developed for the determination of four ß-lactam antibiotics (BLAs) namely cefaclor, cefotaxime, amoxicillin and ampicillin in urine, pharmaceuticals and milk. A mini-column packed with PUF functionalized with the cationic polyelectrolyte, poly(N-chloranil N,N,N',N'-tetramethylethylene diammonium dichloride) PCTDD, was utilized for selective preconcentration. The detection limits with this method were 3.3, 3.8, 5.1 and 7.0 ng mL(-1) and enrichment factors were 38, 21, 39, and 36 for cefaclor, cefotaxime, amoxicillin and ampicillin, respectively with a sample throughput of 12 h(-1) for all BLAs. Moreover, the BLAs were successfully separated by isocratic elution using a micellar mobile phase. Application of the method developed has resulted in recovery values in the range 95%-109% (RSD≤8.7), 83%-99% (RSD≤9.7) and 91%-103% (RSD≤4.0) for urine, pharmaceuticals and milk samples, respectively.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Polímeros
/
Análisis de Inyección de Flujo
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Beta-Lactamas
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Extracción en Fase Sólida
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Métodos Analíticos de la Preparación de la Muestra
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Talanta
Año:
2012
Tipo del documento:
Article
País de afiliación:
Egipto
Pais de publicación:
Países Bajos