[Using of metal-affinity chromatography for identification of alkylated rat globin].

Dubrovskii, Ia A; Gladilovich, V D; Krasnov, I A; Podol'skaia, E P; Murashko, E A; Babakov, V N

Dubrovskii, Ia A; Gladilovich, V D; Krasnov, I A; Podol'skaia, E P; Murashko, E A; Babakov, V N.

Bioorg Khim ; 38(1): 52-7, 2012.

Article en Ru | MEDLINE | ID: mdl-22792705

RESUMEN

Rat globin peptides alkylated by sulfur mustard on amino-acid residues C-126, C-93 and E-27 with MH+ 1444.62 Da, 1561.66 Da, 1676.78 Da, respectively, were concentrated using metal-affinity chromatography on Cu2+. The peptides were received by trypic digestion after in vitro incubation of rat globin with 60 microM HD. Aklylated peptide with MH+ 1444.62 Da is the most sensitive biomarker, which can be concentrated from globin trypic digest, incubated with 3 microM sulfur mustard.

Asunto(s)

Cromatografía de Afinidad/métodos; Hemoglobinas/química; Hemoglobinas/aislamiento & purificación; Gas Mostaza/química; Alquilación; Animales; Ratas

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Hemoglobinas / Cromatografía de Afinidad / Gas Mostaza Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: Ru Revista: Bioorg Khim Año: 2012 Tipo del documento: Article Pais de publicación: Rusia

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