Reversible stress-induced lipid body formation in fast twitch rat myofibers.
Exp Cell Res
; 318(17): 2191-9, 2012 Oct 15.
Article
en En
| MEDLINE
| ID: mdl-22771721
We analyzed the existence of lipid bodies (LBs) in the fast twitch rat flexor digitorum brevis (FDB) myofibers and found that these structures were scarce. However, isolation procedure of the myofibers, heath shock, viral infection or the glycosylation inhibitor tunicamycin induced formation of the LBs, which were stationary structures flanking Z lines. We next infected FDB myofibers with recombinant Semliki Forest virus expressing caveolin 3-yellow fluorescent protein (cav3-YFP) since this chimeric protein was targeted to the LBs facilitating their further analysis. Photobleaching experiments showed that the LBs recovered cav 3-YFP extremely slowly, indicating that they were not continuous with the endoplasmic/sarcoplasmic reticulum. We found, however, that cav3-YFP could move from the LBs to the sarcolemma and this phenomenon was sensitive to Brefeldin A, suggesting that the chimeric protein could be returned from the LBs to the endoplasmic reticulum.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Sarcolema
/
Retículo Sarcoplasmático
/
Fibras Musculares de Contracción Rápida
/
Retículo Endoplásmico
/
Metabolismo de los Lípidos
/
Estrés del Retículo Endoplásmico
Límite:
Animals
Idioma:
En
Revista:
Exp Cell Res
Año:
2012
Tipo del documento:
Article
País de afiliación:
Finlandia
Pais de publicación:
Estados Unidos