The binding of phosphatidylglycerol liposomes to rat platelets is mediated by complement.
Thromb Haemost
; 64(1): 172-6, 1990 Aug 13.
Article
en En
| MEDLINE
| ID: mdl-2274922
Previous work has shown that intravenous administration of phosphatidylglycercol (PG) containing liposomes to rats results in a rapid transient decline in platelet count (1). Here the interactions of PG liposomes with rat platelets in vitro have been examined with the aim of characterizing factors associated with the decline. It is shown that PG liposomes induce formation of rat (but not human) platelet-liposome microaggregates in vitro. The PG liposome dependent thrombocytopenia observed in vivo can therefore be attributed to sequestration of PG liposome-platelet aggregates. Further, the aggregation of platelets with PG liposomes, which can be monitored as a reduction in platelet count using a coulter counter, is shown to be mediated by a serum complement factor, likely C3b. This is indicated by a requirement of plasma for the in vitro reduction in platelet count induced by PG liposomes, and the inhibition of this effect by heat treatment of plasma, by incubation of plasma with purified cobra venom factor, or by removal of C3 from plasma.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Fosfatidilgliceroles
/
Plaquetas
/
Complemento C3
Límite:
Animals
Idioma:
En
Revista:
Thromb Haemost
Año:
1990
Tipo del documento:
Article
País de afiliación:
Canadá
Pais de publicación:
Alemania