Sodium chloride affects propidium monoazide action to distinguish viable cells.
Anal Biochem
; 428(2): 108-10, 2012 Sep 15.
Article
en En
| MEDLINE
| ID: mdl-22728959
Propidium monoazide (PMA) is a DNA-intercalating agent used to selectively detect DNA from viable cells by polymerase chain reaction (PCR). Here, we report that high concentrations (>5%) of sodium chloride (NaCl) prevents PMA from inhibiting DNA amplification from dead cells. Moreover, Halobacterium salinarum was unable to maintain cell integrity in solutions containing less than 15% NaCl, indicating that extreme halophilic microorganisms may not resist the concentration range in which PMA fully acts. We conclude that NaCl, but not pH, directly affects the efficiency of PMA treatment, limiting its use for cell viability assessment of halophiles and in hypersaline samples.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Propidio
/
Azidas
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Cloruro de Sodio
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Halobacterium salinarum
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Viabilidad Microbiana
Idioma:
En
Revista:
Anal Biochem
Año:
2012
Tipo del documento:
Article
País de afiliación:
Brasil
Pais de publicación:
Estados Unidos