The Components of Flemingia macrophylla Attenuate Amyloid ß-Protein Accumulation by Regulating Amyloid ß-Protein Metabolic Pathway.
Evid Based Complement Alternat Med
; 2012: 795843, 2012.
Article
en En
| MEDLINE
| ID: mdl-22719789
Flemingia macrophylla (Leguminosae) is a popular traditional remedy used in Taiwan as anti-inflammatory, promoting blood circulation and antidiabetes agent. Recent study also suggested its neuroprotective activity against Alzheimer's disease. Therefore, the effects of F. macrophylla on Aß production and degradation were studied. The effect of F. macrophylla on Aß metabolism was detected using the cultured mouse neuroblastoma cells N2a transfected with human Swedish mutant APP (swAPP-N2a cells). The effects on Aß degradation were evaluated on a cell-free system. An ELISA assay was applied to detect the level of Aß1-40 and Aß1-42. Western blots assay was employed to measure the levels of soluble amyloid precursor protein and insulin degrading enzyme (IDE). Three fractions of F. macrophylla modified Aß accumulation by both inhibiting ß-secretase and activating IDE. Three flavonoids modified Aß accumulation by activating IDE. The activated IDE pool by the flavonoids was distinctly regulated by bacitracin (an IDE inhibitor). Furthermore, flavonoid 94-18-13 also modulates Aß accumulation by enhancing IDE expression. In conclusion, the components of F. macrophylla possess the potential for developing new therapeutic drugs for Alzheimer's disease.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Idioma:
En
Revista:
Evid Based Complement Alternat Med
Año:
2012
Tipo del documento:
Article
País de afiliación:
Taiwán
Pais de publicación:
Estados Unidos