Evaluation of the Andromas matrix-assisted laser desorption ionization-time of flight mass spectrometry system for identification of aerobically growing Gram-positive bacilli.

Farfour, E; Leto, J; Barritault, M; Barberis, C; Meyer, J; Dauphin, B; Le Guern, A-S; Leflèche, A; Badell, E; Guiso, N; Leclercq, A; Le Monnier, A; Lecuit, M; Rodriguez-Nava, V; Bergeron, E; Raymond, J; Vimont, S; Bille, E; Carbonnelle, E; Guet-Revillet, H; Lécuyer, H; Beretti, J-L; Vay, C; Berche, P; Ferroni, A; Nassif, X; Join-Lambert, O

Farfour, E; Leto, J; Barritault, M; Barberis, C; Meyer, J; Dauphin, B; Le Guern, A-S; Leflèche, A; Badell, E; Guiso, N; Leclercq, A; Le Monnier, A; Lecuit, M; Rodriguez-Nava, V; Bergeron, E; Raymond, J; Vimont, S; Bille, E; Carbonnelle, E; Guet-Revillet, H; Lécuyer, H; Beretti, J-L; Vay, C; Berche, P; Ferroni, A; Nassif, X; Join-Lambert, O.

Afiliación

Farfour E; Université Paris Descartes, Paris, France.

J Clin Microbiol ; 50(8): 2702-7, 2012 Aug.

Article en En | MEDLINE | ID: mdl-22692743

RESUMEN

Matrix-associated laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) is a rapid and simple microbial identification method. Previous reports using the Biotyper system suggested that this technique requires a preliminary extraction step to identify Gram-positive rods (GPRs), a technical issue that may limit the routine use of this technique to identify pathogenic GPRs in the clinical setting. We tested the accuracy of the MALDI-TOF MS Andromas strategy to identify a set of 659 GPR isolates representing 16 bacterial genera and 72 species by the direct colony method. This bacterial collection included 40 C. diphtheriae, 13 C. pseudotuberculosis, 19 C. ulcerans, and 270 other Corynebacterium isolates, 32 L. monocytogenes and 24 other Listeria isolates, 46 Nocardia, 75 Actinomyces, 18 Actinobaculum, 11 Propionibacterium acnes, 18 Propionibacterium avidum, 30 Lactobacillus, 21 Bacillus, 2 Rhodococcus equi, 2 Erysipelothrix rhusiopathiae, and 38 other GPR isolates, all identified by reference techniques. Totals of 98.5% and 1.2% of non-Listeria GPR isolates were identified to the species or genus level, respectively. Except for L. grayi isolates that were identified to the species level, all other Listeria isolates were identified to the genus level because of highly similar spectra. These data demonstrate that rapid identification of pathogenic GPRs can be obtained without an extraction step by MALDI-TOF mass spectrometry.

Asunto(s)

Bacterias Aerobias/química; Bacterias Aerobias/clasificación; Técnicas Bacteriológicas/métodos; Bacterias Grampositivas/química; Bacterias Grampositivas/clasificación; Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción/métodos; Humanos; Sensibilidad y Especificidad; Factores de Tiempo

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bacterias Aerobias / Técnicas Bacteriológicas / Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción / Bacterias Grampositivas Tipo de estudio: Diagnostic_studies / Evaluation_studies / Prognostic_studies Límite: Humans Idioma: En Revista: J Clin Microbiol Año: 2012 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Estados Unidos

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