Structure of the GDP-bound G domain of the RGK protein Rem2.
Acta Crystallogr Sect F Struct Biol Cryst Commun
; 68(Pt 6): 626-31, 2012 Jun 01.
Article
en En
| MEDLINE
| ID: mdl-22684057
RGK proteins are atypical small GTP-binding proteins that are involved in the regulation of voltage-dependent calcium channels and actin cytoskeleton remodelling. The structure of the Rem2 G domain bound to GDP is reported here in a monoclinic crystal form at 2.66 Å resolution. It is very similar to the structure determined previously from an orthorhombic crystal form. However, differences in the crystal-packing environment revealed that the switch I and switch II regions are flexible and not ordered as previously reported. Comparison of the available RGK protein structures along with those of other small GTP-binding proteins highlights two structural features characteristic of this atypical family and suggests that the conserved tryptophan residue in the DXWEX motif may be a structural determinant of the nucleotide-binding affinity.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas de Unión al GTP Monoméricas
/
Dominios y Motivos de Interacción de Proteínas
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Acta Crystallogr Sect F Struct Biol Cryst Commun
Año:
2012
Tipo del documento:
Article
País de afiliación:
Francia
Pais de publicación:
Reino Unido