[Study on extraction and immune regulation of polysaccharides from Marsdenia tenacissima].
Zhongguo Zhong Yao Za Zhi
; 37(4): 457-60, 2012 Feb.
Article
en Zh
| MEDLINE
| ID: mdl-22667143
OBJECTIVE: To optimize the extraction technology and determine the immune activity of polysaccharides from Marsdenia tenacissima. METHOD: The optimum extraction technology of polysaccharide from M. tenacissima was detected by orthogonal experiments with the extraction rate of polysacchride and the total sugar content as indicators. The immunocompromised mice model was established by intraperitoneal injection cyclophosphamide to detected the content of IL-2, IL-6 in serum, CD4+, CD8+ in the peripheral blood by ELISA and flow cytometry, respectively. RESULT: By the extraction rate of polysaccharide, the sequence of seriousness of all affecting factors from high to low was extracting times, temperature, heating time and water ratio. By the total sugar content, the sequence was temperature, extracting times, water ratio and heating time. Compared with the model group, the pleen index, IL-2, IL-6, CD4/CD8+ were increased significantly (P < 0.05) in the 0.14 g x kg(-1) group and 0. 28 g x kg(-1) group. CONCLUSION: T he optimum extraction condition was as follows: extraction three times/1.5 hours at 100 degrees C with 1:8 ratio of M. tenacissima to water. The polysaccharide of M. tenacissima can enhance the cellular immune and humoral immune.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Polisacáridos
/
Marsdenia
/
Fraccionamiento Químico
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
Zh
Revista:
Zhongguo Zhong Yao Za Zhi
Asunto de la revista:
FARMACOLOGIA
/
TERAPIAS COMPLEMENTARES
Año:
2012
Tipo del documento:
Article
País de afiliación:
China
Pais de publicación:
China