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[Determination of streptomycin and dihydrostreptomycin residues in tomato paste by tandem dual solid phase extraction column-liquid chromatography-tandem mass spectrometry].
Gong, Zhiguo; Su, Min; Ji, Xincheng; Li, Shiyu; Wan, Yuping.
Afiliación
  • Gong Z; Technique Center of Xinjiang Entry-Exit Inspection and Quarantine Bureau, Urumqi 830063, China. xjciqgong@163.com
Se Pu ; 30(1): 33-8, 2012 Jan.
Article en Zh | MEDLINE | ID: mdl-22667088
The method was specifically developed for the simultaneous determination of streptomycin and dihydrostreptomycin residues in tomato paste by tandem dual solid phase extraction (SPE) column cleanup-liquid chromatography-tandem mass spectrometry. The residues were extracted from the samples with phosphate buffer solution (pH 4). The cleanup was performed by the way of dispersive solid phase extraction and tandem dual solid phase extraction column. The polar chromatographic column was used to complete the separation of the analytes under gradient elution and the analytes were detected in multiple reaction monitoring (MRM) mode via positive electrospray ionization (ESI +). The external standard calibration curves were used for the quantification. The linear ranges were from 0.01 to 0.2 mg/L with a good linear relationship (r > 0.999) for streptomycin and dihydrostreptomycin. The limit of quantification (LOQs) was 0.02 mg/kg for the both analytes. The recovery range was from 71% to 101% with the relative standard deviations (RSDs) between 2.3% and 15%. It was indicated that this method is accurate, easier, more sensitive, and has a better purification effect in the monitoring and analysis. The method is accurate and specific to monitor and analyze of streptomycin and dihydrostreptomycin residues in tomato paste and its products.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sulfato de Dihidroestreptomicina / Estreptomicina / Cromatografía Liquida / Solanum lycopersicum / Espectrometría de Masas en Tándem Idioma: Zh Revista: Se Pu Año: 2012 Tipo del documento: Article País de afiliación: China Pais de publicación: China
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sulfato de Dihidroestreptomicina / Estreptomicina / Cromatografía Liquida / Solanum lycopersicum / Espectrometría de Masas en Tándem Idioma: Zh Revista: Se Pu Año: 2012 Tipo del documento: Article País de afiliación: China Pais de publicación: China