Genome-wide RNAi screening identifies human proteins with a regulatory function in the early secretory pathway.
Nat Cell Biol
; 14(7): 764-74, 2012 Jun 03.
Article
en En
| MEDLINE
| ID: mdl-22660414
The secretory pathway in mammalian cells has evolved to facilitate the transfer of cargo molecules to internal and cell surface membranes. Use of automated microscopy-based genome-wide RNA interference screens in cultured human cells allowed us to identify 554 proteins influencing secretion. Cloning, fluorescent-tagging and subcellular localization analysis of 179 of these proteins revealed that more than two-thirds localize to either the cytoplasm or membranes of the secretory and endocytic pathways. The depletion of 143 of them resulted in perturbations in the organization of the COPII and/or COPI vesicular coat complexes of the early secretory pathway, or the morphology of the Golgi complex. Network analyses revealed a so far unappreciated link between early secretory pathway function, small GTP-binding protein regulation, actin cytoskeleton organization and EGF-receptor-mediated signalling. This work provides an important resource for an integrative understanding of global cellular organization and regulation of the secretory pathway in mammalian cells.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Vesículas Transportadoras
/
Vesículas Secretoras
/
Interferencia de ARN
/
Endocitosis
/
Redes Reguladoras de Genes
/
Aparato de Golgi
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
/
Screening_studies
Límite:
Humans
Idioma:
En
Revista:
Nat Cell Biol
Año:
2012
Tipo del documento:
Article
País de afiliación:
Irlanda
Pais de publicación:
Reino Unido