PACAP immunoreactivity in human malignant tumor samples and cardiac diseases.

Szanto, Z; Sarszegi, Zs; Reglodi, D; Nemeth, J; Szabadfi, K; Kiss, P; Varga, A; Banki, E; Csanaky, K; Gaszner, B; Pinter, O; Szalai, Zs; Tamas, A

Szanto, Z; Sarszegi, Zs; Reglodi, D; Nemeth, J; Szabadfi, K; Kiss, P; Varga, A; Banki, E; Csanaky, K; Gaszner, B; Pinter, O; Szalai, Zs; Tamas, A.

Afiliación

Szanto Z; Surgery Clinic, University of Pecs, Pecs, Hungary.

J Mol Neurosci ; 48(3): 667-73, 2012 Nov.

Article en En | MEDLINE | ID: mdl-22648511

RESUMEN

Pituitary adenylate cyclase activating polypeptide (PACAP) is a pleiotropic and multifunctional neuropeptide having important roles in various physiological processes. Recent trends in PACAP research point to the clinical introduction of PACAP or its analogs/fragments possibly in the near future. Recently, we have shown the presence of PACAP in human plasma, milk, placenta, and follicular fluid samples. However, relatively few data are available on PACAP in human tissues from patients with different disorders. The aim of the present study was to determine, by radioimmunoassay, the tissue level of PACAP38-like immunoreactivity (LI) and PACAP27-LI in different primary non-small cell lung cancer, colon tumor samples, and in cardiac muscle samples from patients suffering from ischemic heart disease and valvular disorders. We also labeled the PAC1 receptors in human cardiac cells. All samples showed significantly higher PACAP38-LI compared with PACAP27-LI. We found significantly lower levels of PACAP38-LI and PACAP27-LI in tumoral and peripheral samples compared with normal healthy tissue in both lung and colon cancers. Further investigations are necessary to describe the exact function of PACAP in oncogenesis. We showed that PACAP38-LI and PACAP27-LI are significantly higher in ischemic heart diseases compared with valvular abnormalities, suggesting that PACAP might play a role in ischemic heart disorders.

Asunto(s)

Adenocarcinoma/química; Carcinoma de Pulmón de Células no Pequeñas/química; Neoplasias del Colon/química; Enfermedades de las Válvulas Cardíacas/metabolismo; Neoplasias Pulmonares/química; Isquemia Miocárdica/metabolismo; Miocitos Cardíacos/química; Proteínas de Neoplasias/análisis; Polipéptido Hipofisario Activador de la Adenilato-Ciclasa/análisis; Adenocarcinoma/patología; Carcinoma de Pulmón de Células no Pequeñas/patología; Colon/química; Neoplasias del Colon/patología; Enfermedades de las Válvulas Cardíacas/patología; Humanos; Pulmón/química; Neoplasias Pulmonares/patología; Isquemia Miocárdica/patología; Miocardio/química; Polipéptido Hipofisario Activador de la Adenilato-Ciclasa/fisiología; Isoformas de Proteínas/análisis; Radioinmunoensayo; Receptores del Polipéptido Activador de la Adenilato-Ciclasa Hipofisaria/análisis

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Adenocarcinoma / Isquemia Miocárdica / Neoplasias del Colon / Carcinoma de Pulmón de Células no Pequeñas / Miocitos Cardíacos / Polipéptido Hipofisario Activador de la Adenilato-Ciclasa / Enfermedades de las Válvulas Cardíacas / Neoplasias Pulmonares / Proteínas de Neoplasias Límite: Humans Idioma: En Revista: J Mol Neurosci Asunto de la revista: BIOLOGIA MOLECULAR / NEUROLOGIA Año: 2012 Tipo del documento: Article País de afiliación: Hungria Pais de publicación: Estados Unidos

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