Covalently trapping MutS on DNA to study DNA mismatch recognition and signaling.
Mol Biosyst
; 8(7): 1861-4, 2012 Jul 06.
Article
en En
| MEDLINE
| ID: mdl-22627921
The DNA repair protein MutS forms clamp-like structures on DNA that search for and recognize base mismatches leading to ATP-transformed signaling clamps. In this study, the mobile MutS clamps were trapped on DNA in a functional state using single-cysteine variants of MutS and thiol-modified homoduplex or heteroduplex DNA. This approach allows stabilization of various transient MutS-DNA complexes and will enable their structural and functional analysis.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
ADN Bacteriano
/
Proteína MutS de Unión a los Apareamientos Incorrectos del ADN
/
Reparación de la Incompatibilidad de ADN
Idioma:
En
Revista:
Mol Biosyst
Asunto de la revista:
BIOLOGIA MOLECULAR
/
BIOQUIMICA
Año:
2012
Tipo del documento:
Article
País de afiliación:
Alemania
Pais de publicación:
Reino Unido