A method for monitoring antibodies against staphylococcal DNases.
Acta Vet Scand
; 31(2): 223-6, 1990.
Article
en En
| MEDLINE
| ID: mdl-2260516
When small amounts of DNase produced by Staphylococcus aureus, S. intermedius or S. hyicus were added to Toluidine Blue DNA Agar (TDA), a medium for demonstration of staphylococcal antiDNases was produced. By applying this medium in microtitre plates, a test system for titration of staphylococcal antibodies in serum samples was developed. A colour change from blue to pink could be observed when the DNase was allowed to act, i.e. when no staphylococcal antiDNases were present in the samples. When serum with neutralizing antibodies were applied, no colour change developed. An end-point could easily be demonstrated in dilutions of the serum. A description of the method, including certain of its limitations is given.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Staphylococcus
/
Desoxirribonucleasas
/
Nucleasa Microcócica
/
Anticuerpos Antibacterianos
Límite:
Animals
Idioma:
En
Revista:
Acta Vet Scand
Año:
1990
Tipo del documento:
Article
Pais de publicación:
Reino Unido