Characterization of pyrimidine deoxyribonucleoside kinase (thymidine kinase) and thymidylate kinase as a multifunctional enzyme in cells transformed by herpes simplex virus type 1 and in cells infected with mutant strains of herpes simplex virus.
J Virol
; 30(3): 942-5, 1979 Jun.
Article
en En
| MEDLINE
| ID: mdl-225551
Pyrimidine deoxyribonucleoside kinase (thymidine kinase [TK]) was purified from two herpes simplex virus type 1 (HVS-1)-transformed TK-deficient mouse (LMTK-) cell lines and from LMTK- cells infected with HSV-1 mutant viruses coding for variant TK enzymes. These preparations exhibited normal or variant virus-induced thymidylate kinase activities correlating with their relative TK activities. Neither virus-induced activity was detected in LMTK- cells infected with an HSV-1 TK-deficient mutant. These results suggest that HSV-1 thymidylate kinase activity and TK activity are mediated by the same protein.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Fosfotransferasas
/
Timidina Quinasa
/
Transformación Celular Neoplásica
/
Transformación Celular Viral
/
Simplexvirus
/
Nucleósido-Fosfato Quinasa
Límite:
Animals
Idioma:
En
Revista:
J Virol
Año:
1979
Tipo del documento:
Article
Pais de publicación:
Estados Unidos