Surface display of rice stripe virus NSvc2 and analysis of its membrane fusion activity.
Virol Sin
; 27(2): 100-8, 2012 Apr.
Article
en En
| MEDLINE
| ID: mdl-22492001
Rice stripe virus (RSV) infects rice and is transmitted in a propagative manner by the small brown planthopper. How RSV enters an insect cell to initiate the infection cycle is poorly understood. Sequence analysis revealed that the RSV NSvc2 protein was similar to the membrane glycoproteins of several members in the family Bunyaviridae and might induce cell membrane fusion. To conveniently study the membrane fusion activity of NSvc2, we constructed cell surface display vectors for expressing Nsvc2 on the insect cell surface as the membrane glycoproteins of the enveloped viruses. Our results showed that NSvc2 was successfully expressed and displayed on the surface of insect Sf9 cells. When induced by low pH, the membrane fusion was not observed in the cells that expressed NSvc2. Additionally, the membrane fusion was also not detected when co-expressing Nsvc2 and the viral capsid protein on insect cell surface. Thus, RSV NSvc2 is probably different from the phlebovirus counterparts, which could suggest different functions. RSV might enter insect cells other than by fusion with plasma or endosome membrane.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas Virales
/
Membrana Celular
/
Tenuivirus
/
Internalización del Virus
Límite:
Animals
Idioma:
En
Revista:
Virol Sin
Asunto de la revista:
VIROLOGIA
Año:
2012
Tipo del documento:
Article
País de afiliación:
China
Pais de publicación:
Países Bajos