A crucial role for Mim2 in the biogenesis of mitochondrial outer membrane proteins.
J Cell Sci
; 125(Pt 14): 3464-73, 2012 Jul 15.
Article
en En
| MEDLINE
| ID: mdl-22467864
Most of the mitochondrial outer membrane (MOM) proteins contain helical transmembrane domains. Some of the single-span proteins and all known multiple-span proteins are inserted into the membrane in a pathway that depends on the MOM protein Mitochondrial Import 1 (Mim1). So far it has been unknown whether additional proteins are required for this process. Here, we describe the identification and characterization of Mim2, a novel protein of the MOM that has a crucial role in the biogenesis of MOM helical proteins. Mim2 physically and genetically interacts with Mim1, and both proteins form the MIM complex. Cells lacking Mim2 exhibit a severely reduced growth rate and lower steady-state levels of helical MOM proteins. In addition, absence of Mim2 leads to compromised assembly of the translocase of the outer mitochondrial membrane (TOM complex), hampered mitochondrial protein import, and defects in mitochondrial morphology. In summary, the current study demonstrates that Mim2 is a novel central player in the biogenesis of MOM proteins.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas de Saccharomyces cerevisiae
/
Membranas Mitocondriales
Idioma:
En
Revista:
J Cell Sci
Año:
2012
Tipo del documento:
Article
País de afiliación:
Alemania
Pais de publicación:
Reino Unido