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[Pronase E and P immobilization on aminoorganosilica surface]. / Immobilizatsiia pronazy e i p na poverkhnosti aminoorganokremiezema.
Ukr Biokhim Zh (1978) ; 51(4): 315-8, 1979.
Article en Uk | MEDLINE | ID: mdl-224541
Immobilization of pronase E and P was performed on sylochrome modified by gamma-aminopropyltrietoxysilane using cyanuric chloride, 2,4--toluylenediisocyanate, glutaric aldehyde and also on sylochrome by means of titanium tetrachloride. The esterase and caséinolytic activities of the immobilized preparations and their stability during storage were determined. The increased thermostability of the immobilized preparation is established. The influence of the enzyme: carrier ratio on the immobilization process and activity of the enzymes was studied. It is shown that application of glutaric aldehyde favours the best retention of the esterase activity (73%), whereas the caseinolytic activity is better retained (31%) when titanium tetrachloride was used.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pronasa / Enzimas Inmovilizadas Idioma: Uk Revista: Ukr Biokhim Zh (1978) Año: 1979 Tipo del documento: Article Pais de publicación: Ucrania
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pronasa / Enzimas Inmovilizadas Idioma: Uk Revista: Ukr Biokhim Zh (1978) Año: 1979 Tipo del documento: Article Pais de publicación: Ucrania