Time-course analysis of injured skeletal muscle suggests a critical involvement of ERK1/2 signaling in the acute inflammatory response.
Muscle Nerve
; 45(4): 552-61, 2012 Apr.
Article
en En
| MEDLINE
| ID: mdl-22431089
INTRODUCTION: The coupling and timing of pro- and anti-inflammatory processes in skeletal muscle injury is poorly understood. We investigated the temporal response and regulated processes of extracellular signal-regulated kinases 1 and 2 (ERK1/2), p38, and IkappaB kinase (IKK) α/ß signaling pathways after traumatic injury. METHODS: Traumatic freeze injury was delivered to the tibialis anterior (TA) muscle in C57BL/6J mice, and injured and uninjured TA muscles were analyzed 3-72 h into the recovery period. RESULTS: Significant increases in pro-inflammatory cytokine transcription accompanied IKKß phosphorylation, robust ERK pathway activation, and reduced heat shock protein (Hsp) protein expression at 3-24 h. At 24 h, ERK activation was abolished concomitantly with a significant increase in mitogen-activated protein kinase phosphatase-1 (MKP-1). After 24 h, cytokine transcription along with ERK1/2 and IKKß phosphorylation remained suppressed, whereas Hsp protein expression rose to significant levels by 72 h and associated with IKKß. CONCLUSIONS: Results indicate a bimodal regulation of ERK1/2 in acute inflammation in which it is supportive from 3 to 24 h, and suppressive from 24 to 72 h.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Músculo Esquelético
/
Sistema de Señalización de MAP Quinasas
/
Inflamación
Límite:
Animals
Idioma:
En
Revista:
Muscle Nerve
Año:
2012
Tipo del documento:
Article
País de afiliación:
Estados Unidos
Pais de publicación:
Estados Unidos