Biological characterization of Nigerian chicken anaemia virus isolates.
Afr J Med Med Sci
; 39 Suppl: 21-6, 2010 Dec.
Article
en En
| MEDLINE
| ID: mdl-22416640
Chicken anaemia virus (CAV) DNA was extracted from thymus, liver and bone marrow samples obtained from broiler and pullet chicken flocks in southwestern Nigeria, which presented with clinical signs and lesions suggestive of both infectious bursal disease and chicken infectious anaemia. While CAV was successfully isolated in MDCC-MSB1 cells from four of the pooled tissue samples, the remaining two samples failed to grow in cells. Monoclonal antibody (MAb) characterization using four MAbs produced against the reference Cuxhaven-1 (Cux-1) CAV isolate showed that Nigerian CAV isolates are antigenically related to each other and to the Cux-1 virus. Pathogenicity studies with the Cux-1 virus and one of the Nigerian isolates (NGR-1) revealed that NGR-1 was more pathogenic that the former. We conclude that although Nigerian CAV isolates are antigenically related to each other, they differ in terms of cell culture growth characteristics and probably pathogenicity. These findings further confirm that CAV exists and can no longer be ignored in poultry disease diagnosis in Nigeria. Cases hitherto diagnosed as IBD may actually be CIA or a co-infection of the two.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Enfermedades de las Aves de Corral
/
Pollos
/
Infecciones por Circoviridae
/
Virus de la Anemia del Pollo
Límite:
Animals
País/Región como asunto:
Africa
Idioma:
En
Revista:
Afr J Med Med Sci
Año:
2010
Tipo del documento:
Article
País de afiliación:
Nigeria
Pais de publicación:
Nigeria