[Effect of BYDV-MP nuclear localization signal on the movement of PVX].
Bing Du Xue Bao
; 28(1): 35-44, 2012 Jan.
Article
en Zh
| MEDLINE
| ID: mdl-22416348
Abstract:By using PVX derived vector pGR107, the effect of BYDV-MP nuclear localization signal on the movement of PVX was studied. BYDV-MP was cloned into pGR107 using GFP as an indicator. BYDV-MP was then shown to induce the systemic infection and exacerbate the symptom of PVX through infecting Nicotiana benthamiana. When the PVX gene encoding 25kD protein, which functioned as a systematic movemnet protein,was deleted and the above experiment was repeated, the result showed that BYDV-MP could compensate the systemic movement of PVX. A serial mutants with substitutions on the fifth, sixth and seventh amino acids of BYDV-MP nuclear localization signal was further constructed. It was found that the mutants at the fifth, sixth amino acids in BYDV-MP nuclear localization signal could only delay or weaken systemic movement of PVX whereas the mutant at seventh amino acid could entirely inhibit systemic movement of PVX.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Potexvirus
/
Luteovirus
/
Señales de Localización Nuclear
/
Proteínas de Movimiento Viral en Plantas
Idioma:
Zh
Revista:
Bing Du Xue Bao
Asunto de la revista:
VIROLOGIA
Año:
2012
Tipo del documento:
Article
País de afiliación:
China
Pais de publicación:
China