Cytotropism of Theiler's murine encephalomyelitis viruses in oligodendrocyte-enriched cultures.
Arch Virol
; 114(3-4): 293-8, 1990.
Article
en En
| MEDLINE
| ID: mdl-2241579
The cytotropism of two strains, GDVII and DA, of Theiler's murine encephalomyelitis viruses (TMEV) was studied in the oligodendrocyte-enriched murine neural cell cultures. Both GDVII and DA caused cytopathic effects in the neural cell cultures, and double immunostaining for galactocerebroside (Gal-Cer), a marker molecule for oligodendrocyte, and viral antigens disclosed a dual expression of Gal-Cer and viral antigens in over 80% of cells in both cultures 24 h after infection with either GDVII or DA. The kinetics of cell-free and cell-associated infectivity were not significantly different between two cultures. These in vitro observations suggest that neither replication in oligodendrocyte nor cell-associated infectivity is a sole factor in discriminating those two subgroups of TMEV with regard to the demyelinating activity, and that virus cell binding may play an important role in virus persistence and TMEV-induced demyelination.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Virus Maus Elberfeld
/
Neuronas
Límite:
Animals
Idioma:
En
Revista:
Arch Virol
Año:
1990
Tipo del documento:
Article
País de afiliación:
Japón
Pais de publicación:
Austria