Can we see PIP(3) and hydrogen peroxide with a single probe?
Antioxid Redox Signal
; 17(3): 505-12, 2012 Aug 01.
Article
en En
| MEDLINE
| ID: mdl-22369174
A genetically encoded sensor for parallel measurements of phosphatidylinositol 3-kinase activity and hydrogen peroxide (H(2)O(2)) levels (termed PIP-SHOW) was developed. Upon elevation of local phosphatidylinositol 3,4,5-trisphosphate (PIP(3)) concentration, the sensor translocates from the cytosol to the plasma membrane, while a ratiometric excitation change rapidly and simultaneously reports changes in the concentration of H(2)O(2). The dynamics of PIP(3) and H(2)O(2) generation were monitored in platelet-derived growth factor-stimulated fibroblasts and in T-lymphocytes after formation of an immunological synapse. We suggest that PIP-SHOW can serve as a prototype for many fluorescent sensors with combined readouts.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas Bacterianas
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Proteínas Recombinantes de Fusión
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Proteínas Tirosina Quinasas
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Fosfatos de Fosfatidilinositol
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Colorantes Fluorescentes
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Peróxido de Hidrógeno
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Proteínas Luminiscentes
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Antioxid Redox Signal
Asunto de la revista:
METABOLISMO
Año:
2012
Tipo del documento:
Article
País de afiliación:
Rusia
Pais de publicación:
Estados Unidos