Improvement of D-lactate productivity in recombinant Escherichia coli by coupling production with growth.
Biotechnol Lett
; 34(6): 1123-30, 2012 Jun.
Article
en En
| MEDLINE
| ID: mdl-22367280
Coupling lactate fermentation with cell growth was investigated in shake-flask and bioreactor cultivation systems by increasing aeration to improve lactate productivity in Escherichia coli CICIM B0013-070 (ackA pta pps pflB dld poxB adhE frdA). In shake-flasks, cells reached 1 g dry wt/l then, cultivated at 100 rpm and 42°C, achieved a twofold higher productivity of lactic acid compared to aerobic and O(2)-limited two-phase fermentation. The cells in the bioreactor yielded an overall volumetric productivity of 5.5 g/l h and a yield of 86 g lactic acid/100 g glucose which were 66% higher and the same level compared to that of the aerobic and O(2)-limited two-phase fermentation, respectively, using scaled-up conditions optimized from shake-flask experiments. These results have revealed an approach for improving production of fermentative products in E. coli.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Ácido Láctico
/
Escherichia coli
/
Ingeniería Metabólica
Idioma:
En
Revista:
Biotechnol Lett
Año:
2012
Tipo del documento:
Article
Pais de publicación:
Países Bajos