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Bromelain ameliorates the wound microenvironment and improves the healing of firearm wounds.
Wu, Si-Yu; Hu, Wei; Zhang, Bo; Liu, Shuai; Wang, Jian-Min; Wang, Ai-Min.
Afiliación
  • Wu SY; Department of Orthopaedics, Daping Hospital, Third Military Medical University, Chongqing, China.
J Surg Res ; 176(2): 503-9, 2012 Aug.
Article en En | MEDLINE | ID: mdl-22341346
BACKGROUND: In a previous study, we proposed a new therapy using topical bromelain as a supplement to simple wound-track incision for the debridement of firearm wounds. This enzymatic debridement greatly simplified the management of high-velocity gunshot wounds in a pig model, and bromelain was confirmed to improve wound healing. The purpose of the present study was to investigate the effect of bromelain on the microenvironment of firearm wounds. METHODS: Sixteen Chinese landrace pigs wounded by high-velocity projectiles were divided randomly into four groups: wound incision (group I), incision + bromelain (group IB), wound excision (group E), and control. Blood perfusion, oxygen partial pressure (pO(2)), and the content of tumor necrosis factor (TNF)-α and transforming growth factor (TGF)-ß in wound-track tissue were measured. Wound healing was also noted. RESULTS: The recovery of blood perfusion in tissue and pO(2) in wound tracks was significantly more rapid in group IB and group E than in group I and control. The tissue level of TNF-α was significantly lower in group IB than in group I and control 48 h and 72 h post-wounding, and was lower than in group E 48 h post-wounding. The tissue level of TGF-ß in group IB was sustained at a significantly higher level than in the other three groups. Wound healing time was also shorter in group IB. CONCLUSIONS: Enzymatic debridement using topical bromelain in incised wound tracks accelerates the recovery of blood perfusion, pO(2) in wound tissue, controls the expression of TNF-α and raises the expression of TGF-ß.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cicatrización de Heridas / Heridas por Arma de Fuego / Bromelaínas / Desbridamiento / Ananas / Miembro Posterior Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: J Surg Res Año: 2012 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cicatrización de Heridas / Heridas por Arma de Fuego / Bromelaínas / Desbridamiento / Ananas / Miembro Posterior Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: J Surg Res Año: 2012 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos