Identification of candidate genes underlying an iron efficiency quantitative trait locus in soybean.

Peiffer, Gregory A; King, Keith E; Severin, Andrew J; May, Gregory D; Cianzio, Silvia R; Lin, Shun Fu; Lauter, Nicholas C; Shoemaker, Randy C

Peiffer, Gregory A; King, Keith E; Severin, Andrew J; May, Gregory D; Cianzio, Silvia R; Lin, Shun Fu; Lauter, Nicholas C; Shoemaker, Randy C.

Afiliación

Peiffer GA; Department of Agronomy, Iowa State University, Ames, Iowa 50010, USA.

Plant Physiol ; 158(4): 1745-54, 2012 Apr.

Article en En | MEDLINE | ID: mdl-22319075

RESUMEN

Prevalent on calcareous soils in the United States and abroad, iron deficiency is among the most common and severe nutritional stresses in plants. In soybean (Glycine max) commercial plantings, the identification and use of iron-efficient genotypes has proven to be the best form of managing this soil-related plant stress. Previous studies conducted in soybean identified a significant iron efficiency quantitative trait locus (QTL) explaining more than 70% of the phenotypic variation for the trait. In this research, we identified candidate genes underlying this QTL through molecular breeding, mapping, and transcriptome sequencing. Introgression mapping was performed using two related near-isogenic lines in which a region located on soybean chromosome 3 required for iron efficiency was identified. The region corresponds to the previously reported iron efficiency QTL. The location was further confirmed through QTL mapping conducted in this study. Transcriptome sequencing and quantitative real-time-polymerase chain reaction identified two genes encoding transcription factors within the region that were significantly induced in soybean roots under iron stress. The two induced transcription factors were identified as homologs of the subgroup lb basic helix-loop-helix (bHLH) genes that are known to regulate the strategy I response in Arabidopsis (Arabidopsis thaliana). Resequencing of these differentially expressed genes unveiled a significant deletion within a predicted dimerization domain. We hypothesize that this deletion disrupts the Fe-DEFICIENCY-INDUCED TRANSCRIPTION FACTOR (FIT)/bHLH heterodimer that has been shown to induce known iron acquisition genes.

Asunto(s)

Genes de Plantas/genética; Estudios de Asociación Genética; Glycine max/genética; Glycine max/metabolismo; Hierro/metabolismo; Sitios de Carácter Cuantitativo/genética; Cromosomas de las Plantas/genética; Cruzamientos Genéticos; Perfilación de la Expresión Génica; Regulación de la Expresión Génica de las Plantas; Marcadores Genéticos; Endogamia; Repeticiones de Microsatélite/genética; Modelos Moleculares; Anotación de Secuencia Molecular; Fenotipo; Mapeo Físico de Cromosoma; Reacción en Cadena en Tiempo Real de la Polimerasa; Recombinación Genética/genética; Análisis de Secuencia de ADN; Factores de Transcripción/genética; Factores de Transcripción/metabolismo

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Glycine max / Genes de Plantas / Sitios de Carácter Cuantitativo / Estudios de Asociación Genética / Hierro Tipo de estudio: Diagnostic_studies / Prognostic_studies Idioma: En Revista: Plant Physiol Año: 2012 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos

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