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A photosynthetic biosensor with enhanced electron transfer generation realized by laser printing technology.
Touloupakis, Eleftherios; Boutopoulos, Christos; Buonasera, Katia; Zergioti, Ioanna; Giardi, Maria Teresa.
Afiliación
  • Touloupakis E; Institute of Crystallography, National Research Council, Rome, Italy. toulou_e@chemistry.uoc.gr
Anal Bioanal Chem ; 402(10): 3237-44, 2012 Apr.
Article en En | MEDLINE | ID: mdl-22302172
One of the limits of current electrochemical biosensors is a lack of methods providing stable and highly efficient junctions between biomaterial and solid-state devices. This paper shows how laser-induced forward transfer (LIFT) can enable efficient electron transfer from photosynthetic biomaterial immobilized on screen-printed electrodes (SPE). The ideal pattern, in terms of photocurrent signal of thylakoid droplets giving a stable response signal with a current intensity of approximately 335 ± 13 nA for a thylakoid mass of 28 ± 4 ng, was selected. It is shown that the efficiency of energy production of a photosynthetic system can be strongly enhanced by the LIFT process, as demonstrated by use of the technique to construct an efficient and sensitive photosynthesis-based biosensor for detecting herbicides at nanomolar concentrations.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Extractos Vegetales / Técnicas Biosensibles / Tilacoides / Herbicidas Idioma: En Revista: Anal Bioanal Chem Año: 2012 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Alemania

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Extractos Vegetales / Técnicas Biosensibles / Tilacoides / Herbicidas Idioma: En Revista: Anal Bioanal Chem Año: 2012 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Alemania