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Selective uncoupling of individual mitochondria within a cell using a mitochondria-targeted photoactivated protonophore.
Chalmers, Susan; Caldwell, Stuart T; Quin, Caroline; Prime, Tracy A; James, Andrew M; Cairns, Andrew G; Murphy, Michael P; McCarron, John G; Hartley, Richard C.
Afiliación
  • Chalmers S; Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, 161 Cathedral Street, Glasgow G4 0RE, UK.
J Am Chem Soc ; 134(2): 758-61, 2012 Jan 18.
Article en En | MEDLINE | ID: mdl-22239373
Depolarization of an individual mitochondrion or small clusters of mitochondria within cells has been achieved using a photoactivatable probe. The probe is targeted to the matrix of the mitochondrion by an alkyltriphenylphosphonium lipophilic cation and releases the protonophore 2,4-dinitrophenol locally in predetermined regions in response to directed irradiation with UV light via a local photolysis system. This also provides a proof of principle for the general temporally and spatially controlled release of bioactive molecules, pharmacophores, or toxins to mitochondria with tissue, cell, or mitochondrion specificity.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Compuestos Organofosforados / Mitocondrias Límite: Animals Idioma: En Revista: J Am Chem Soc Año: 2012 Tipo del documento: Article Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Compuestos Organofosforados / Mitocondrias Límite: Animals Idioma: En Revista: J Am Chem Soc Año: 2012 Tipo del documento: Article Pais de publicación: Estados Unidos